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Molecular Biology Reagents
> Enzymes for Molecular Biology
BIOTAQ DNA polymerase 500U
BIO TAQ is a high-performance thermostable DNA polymerase, isolated from Thermus aquaticus YT-1 as described by Kaledin (2). BIOTAQ is a robust enzyme with very high activity, which produces excellent yields in primer extension and other molecular biology applications. BIOTAQ is specially purified to remove traces of DNA that can interfere with some reactions. BIOTAQ is supplied with 10x NH4 -based reaction buffer, (that provides superior results with minimal background,) and a separate solution of 50mM MgCl2, which allows users to tailor-make reaction conditions to particular needs. BIOTAQ has outstanding activity - some users find that as little as 0.1 unit is enough for their reactions - which makes BIOTAQ one of the best value polymerases on the market.
， Excellent yields ， Highly purified ， Superb value
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72ºC under the following assay conditions: 25mM TAPS (tris-[hydroxymethyl]methyl-amino-propanesulphonic acid, sodium salt), pH 9.3 at 25ºC, 50mM KCl, 2mM MgCl2, 1mM b-mercaptoethanol, 200µM of each dATP, dGTP, dTTP, 100µM dCTP (a mix of unlabelled and g-[32P]-labelled) and 12.5µg activated salmon sperm DNA in a final volume of 50µl.
20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% glycerol, 0.1% Tween-20.
(10x)160mM (NH4)2 SO4 , 670mM Tris-HCl (pH 8.8 at 25ºC), 0.1% Tween-20.
Separate Magnisium Solutions
50mM MgCl2 .
Quality Control Tests
Activity, non-specific endonucleases/nickase, exonucleases
BIOTAQ will remain stable at -20ºC in a constant-temperature freezer