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--- New 8-OHdG Check ---

 

8-OHdG Check is a competitive enzyme-linked immunosorbent assay for quantitative measurement of the oxidative DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA in tissue, urine and so on.

The strong feature of 8-OHdG Check is:

1.	Easy operation:	There is no need for expensive equipments and sample pretreatment.
2.	Highly sensitive:	Monoclonal antibody recognizes 8-OHdG specifically (21 analogues of 8-OHdG do not have any cross reactivity).
3.	Speedy:	Estimated time of assay is 3.5 to 4 hours.
4.	Sample capacity of one kit with blank and standard is 18 for triplicate samples. Only 150µl of sample is required (50µl per well).
5.	The use of split type microplate prevents the waste of test samples.

 

Merits of "8-OHdG Check" Kit

The "8-OHdG Check" is useful as a non-invasive indicator in various fields, such as

1. In a primary evaluation of individual life style; in general, to learn whether individual antioxidant system are controlling properly excess active oxygen generated inside body and whether the oxidative stress is restrained within manageable limit, so that whether individual life style is in a state to prevent from diseases and to aging to enjoy the individual maximum life span.
2. In assessing the effect of antioxidant substance (food) in vivo.
3. In evaluating how much oxidative stress is generated by exercises performed or specific activities to which body is subjected to.
4. In studying the correlation between disease and oxidative stress.
5. In determining the side effect of oxidative stress following a specific clinical treatment procedure or pharmaceutical dose of medicine.

* Such techniques as HPLC-EC and LC-MS/MS which, by the nature of their procedure, measure only free form of 8-OHdG in biological samples, and can not detect 8-OHdG contained with in oligomers or 8-OHdG derivatives such as 8-OHdG holding SO₃^- or Glucuronic acid. ELISA technique is preferable to detect over all oxidative stress level in side body. (Referred by M.S.Cooke etc. Free Radical Research 36(9) pp929-932, 2002 etc.)

Kit Contents

1. 8-OHdG coated microtiter plate (split type)
2. Primary antibody (Anti-8-OHdG monoclonal antibody)
3. Primary antibody solution
4. Secondary antibody (POD-conjugated anti mouse antibody)
5. Secondary antibody solution
6. Chromatic solution (3,3',5,5'-tetramethylbenzidine)
7. Substrate solution
8. Washing solution
9. Reaction terminating solution
10. Standard 8-OHdG solution (0.5, 2, 8, 20, 80, 200 ng/ml)
11. Plate seal

Assay Procedure

(1) Primary Antibody Reaction (Competitive Reaction): 37°C for 1 hour

(2) Secondary Antibody Reaction: 37°C for 1 hour

(3) Development of Color Reaction: Room Temperature for 15 min in the dark

(4) Absorbance Reading (wavelength at 450 nm) and Calculation of Results