Specifications and Use

Description

l          Recombinant human IL-11 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids, and having a predicted molecular mass of approximately 19.0kD, but migrates in SDS-PAGE with an apparent molecular mass of 20.8kD.

Source

l          E. coli

Molecular Mass

l          Approximately 19.0kD.

 

Purity

l          ≥97%, as determined by SDS-PAGE and HPLC method.

Endotoxin Level

l          ≤1EU/µg, determined by the LAL method.

Biological Activity

l        Measured in a cell proliferation assay using B₉-11, the specific activity shall be not less than 8×10⁶U/mg.
 

Formulation

l          Lyophilized from a 0.2µm filtered solution in 10mM Phosphate Buffer.

 

Reconstitution

l        It is recommended that sterile ddH₂O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 10µg/mL of the cytokine.

 

Storage

l          Lyophilized samples are stable for greater than six months from date of receipt at -20℃ to -70℃.

l          Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

l          Avoid repeated freeze-thaw cycles.