|
|
|
Total RNA Purification Kit |
|
|
Name:
|
Total RNA Purification Kit |
|
Type:
|
110210 |
|
Message:
|
Total RNA Purification Kit provides a
rapid, safe and reliable way to purify total RNA from a wide
variety of cells and tissues. The purified total RNA is free
from contaminants, essentially is of full length and suitable
for all applications of RNA.
Features
No phenol/chloroform extraction, no use of ethidium bromide and
no CsCldensity gradient ultracentrifugation.
Extracted RNA is free from contaminants, easy to be redissolved
in water or Tris buffer.
RNases in cells are rapidly inactivated.
High recovery of pure total RNA.
Principle
Buffer R-A containing guanidine iso-thiocyanate, a strong
protein denaturant, lyses cells and releases total RNA into the
supernatant. Meanwhile, Buffer R-A inactivates cellular RNases
immediately to protect RNA from degradation. Addition of Buffer
R-B to homogenate results in denaturation of protein, which
forms insoluble clumps with genomic DNA. Proteins are further
precipitated by the unique two-phase partition formed by
addition of Buffer R-C. In this two-phase system RNA remains
soluble and is separated into the lower phase. RNA is
selectively precipitated when second two-phase partition is
performed by addition of Buffer R-D into the lower phase.
However, free genomic DNA remains soluble in lower phase and is
separated from RNA when RNA is precipitated by centrifugation.
Trace impurity is removed by second RNA precipitation performed
in two-phase partition with Buffer R-E and Buffer R-F.
Procedure
Sample is homogenized in Buffer R-A. Add Buffer R-B to
precipitate proteins. After addition of Buffer R-C, solution
turns in to two phases and proteins are co-precipitated with
genomic DNA and pelleted on the interface of the two phases and
total RNA remains soluble and enters into the lower phase.
Transfer the lower phase to a new microfuge tube and add Buffer
R-D to form second two-phase system. In this step RNA is
selectively precipitated from the lower phase, but sheared free
genomic DNA, mitochondria l DNA, viral DNA or transfected
vectors remain in the lower phase and are removed. The total RNA
pellet is redissolved in Buffer R-A. Add buffer R-E and Buffer
R-F for additional RNA precipitation wit h two-phase partition.
The RNA pellet is then dissolved in H2O or Tris buffer and can
be used for all RNA applications.
Applications
After first precipitation by Buffer R-D, the purified total RNA
is suitable for RNA analysis experiments as follows:
Northern, dot or slot blotting
RNA protection detection
In vitro translation
DNA microarray-based analysis
Poly A+ RNA selection
After re-precipitated by Buffer R-F, the purified total RNA can
be used for any RNA applications including RT-PCR and cDNA
library construction.
|
|
Standard:
|
Cat. No.
------------------110210-04-----------------------------110210-08
Sample size-------4X108 cells/4 g tissues------------8X108
cells/8 g tissues
|
|
|
|
|
|
Parallel Genomic DNA & RNA Purification Kit |
|
|
Name:
|
Parallel Genomic DNA & RNA Purification Kit |
|
Type:
|
110250 |
|
Message:
|
Parallel Genomic DNA & RNA Purification Kit
provides a rapid way to obtain pure RNA and genomic DNA from the same
sample.
Features
No phenol/chloroform extraction, no CsCl density gradient
ultracentrifugation.
Isolate pure RNA and DNA from same sample.
Principle and Procedure
The total RNA is released and isolated with two-phase partition as
described in Total RNA Purification Kit. Genomic DNA remaining in the lower
phase and precipitated on the interface of two phases is dissolved in Buffer
G-A and selectively binds to silica membrane. Impurities are removed by
washing with Buffer W1 and Buffer W2. Pure DNA is eluted in small volume of
water or low-salt Tris buffer.
Applications
The total RNA is suitable for Northern blotting, RNA protection detection,
in vitro translation, RT-PCR, cDNA library construction, etc. The genomic
DNA can be applied in Southern blotting, PCR, RAPD, AFLP or RFLP, apoptosis
analysis, etc.
|
|
Standard:
|
Cat. No.
-----------------------------------110250-25-------------------------110250-50
Sample size--------------------2.5X108 cells/2.5 g tissue----------5X108
cells/5 g tissue
|
|
|
 
send mail to [email protected] with questions or comments about this web site.
Copyright © 2008 Gentaur Molecular Products
Site powered by Acid Dragon (AC)
Last modified:
05/19/16
|
+32 1658 9045
+32 1650 9045